A day after Supreme Court said it could not give its verdict on jallikattu before Pongal, DMK pressed the Centre to promulgate an ordinance to allow the sport.
In Karisalkulam village near Madurai, the sport was held in an open ground for a few minutes, a police official said.
In a symbolic protest, around five bulls were let into the ground by a group of youths, he said, adding no arrests were made.
In Coimbatore district, a group of 150 villagers conducted rekla race in Ettimadi village protesting the jallikkattu ban. No arrests were made, police said.
The acts of defiance came against the background of a similar show yesterday in Cuddalore where 28 persons were arrested for going ahead with the sport despite the ban.
DMK Working President M K Stalin led a protest in Chennai and sought promulgation of an ordinance to allow holding the sport.
Targeting Prime Minister Narendra Modi, Stalin, the Leader of the Opposition in the state assembly, wondered why he cannot give time to AIADMK MPs to discuss an important heritage issue of Tamilians when he could meet actors.
Elsewhere in the state, protests by students and other pro-Tamil groups were held demanding that the sport be allowed and for "banning" PETA which, according to them, was acting against the interests of Tamil Nadu.
A group of jallikattu supporters briefly picketed locale of the shooting of Tamil film "Garjanai", starring Trisha, in Sivaganga district, angered by the actor's pro-PETA stand.
Meanwhile, heavy police force have been deployed in Avaniapuram, Palamedu and Alanganallur, famous locations for jallikattu in Madurai District, as various organisations vowed to violate the ban and organise the sport.
Police said all steps have been taken to ensure maintenance of law and order and compliance of court's ban even as district officials said so far no decision had been taken on promulgating prohibitory orders in these places.
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