It was being rumoured that the 35-year-old actress was dating Segal, 34, after splitting from her former husband Tom Cruise in 2012, reported Us magazine.
Holmes and Segel had met on the set of his hit sitcom 'How I Met Your Mother' in 2011, when she had guest starred on the show
"The story is completely untrue. The two have not spoken since she appeared on 'How I Met Your Mother'," Holmes' representative Leslie Sloane, said.
Holmes was most recently linked to Jamie Foxx, while Segel parted ways with Michelle Williams in February, 2013 after a year of dating.
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