Fresh from three days of talks in Switzerland, Kerry gathered at a Riyadh air base with foreign ministers from the six Gulf Cooperation Council nations.
Their agenda is also expected to include ways of reinforcing the battle against jihadists in Iraq and Syria.
Kerry arrived after talks with Iran's Foreign Minister Mohammad Javad Zarif, aiming to seal a nuclear deal with Tehran ahead of a March 31 deadline.
Sunni Gulf nations remain wary about the growing rapprochement between Shiite-dominated Iran and Washington.
"For all the objections that any country has to Iranian activities in the region -- and believe me, we have objections and others in the world have objections -- the first step is to prevent Iran from acquiring a nuclear weapon," he told reporters yesterday before leaving Switzerland.
Iran has provided military assistance to Syria to fight anti-regime forces and to Iraq for the battle against Sunni extremists.
It is also accused of backing Huthi Shiite militiamen who have seized the capital in Saudi Arabia's neighbour, Yemen, and paralysed the Western-back government.
"This is not about a broader rapprochement in any way. This is about the nuclear issue and that's it," she added.
The so-called P5+1 group of Britain, China, France, Russia, the US and Germany is trying to strike an accord that would prevent Tehran -- Riyadh's regional rival -- from developing a nuclear bomb.
In return, the West would ease punishing sanctions on Iran, which insists its nuclear programme is purely civilian.
Saudi Arabia and other Gulf nations belong to an international coalition brought together by the US to fight Sunni militants from the Islamic State (IS) group, which has captured a swathe of territory in Iraq and Syria.
The kingdom has agreed to launch with the US a facility for training and equipping vetted members of the moderate armed opposition from Syria, under a long-planned effort to take on the IS militants.
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