The pair are scheduled to address approximately 300 city leaders from around the country, reported Entertainment Weekly.
According to a release, the Dalai Lama will "focus on the global significance of building kind and compassionate cities through action at the local level."
After his remarks, Gaga, 30, will have an onstage conversation and Q&A. Former NBC News anchor Ann Curry will moderate the discussion.
Ahead of their address, Gaga retweeted one of the Dalai Lama's messages on Twitter.
The "pokerface" hitmaker is the co-founder of the "Born This Way Foundation", which also encourages young people to create a kinder and braver world.
Hillary Clinton will also speak to the group at the event.
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