The 302-foot- (92-meter-) long Airlander 10 was due to take off from an airfield 73 kilometers north of London on the first in a series of test flights.
Stephen McGlennan, chief executive of Hybrid Air Vehicles, said the airship had "a slight technical issue" and there's not time to fix it before darkness fell.
He didn't say when the flight might be rescheduled.
Nicknamed the "flying bum" because of its bulbous front end, the Airlander is a hybrid air vehicle, part lighter-than-air blimp, part plane.
Its developers say it can travel at up to 148 kph and stay aloft for up to two weeks.
The aircraft was initially developed for the US military, which planned to use it for surveillance in Afghanistan.
The US blimp program was scrapped in 2013, and since then the airship's developer has sought funding from government agencies and individual donors.
The vast aircraft is based at Cardington airfield, where the first British airships were built during and after World War I.
The program was abandoned after a 1930 crash that killed almost 50 people, including Britain's air minister.
Unlike hydrogen, the gas used in the Hindenburg, helium is not flammable.
Speaking today before the technical hitch, McGlennan said he was confident airships have a strong future, despite their public image as a failed 20th-century aviation experiment.
"It's a disruptive capability," he said, likening it to the electric car a technology finally making a long-elusive breakthrough into mass use thanks to Elon Musk's Tesla.
"Something that disruptive, it's always long, and it's always a winding road," McGlennan said.
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