Mary Kom (51kg), who was returning to action after a one-year hiatus, went down by a unanimous decision to Korean Chol Mi Bang.
The 34-year-old, a five-time world champion and an Olympic bronze-medallist, struggled to connect clean punches against her taller rival and was even warned once for excessive bending.
Mary Kom would be going back to the light flyweight 48kg category after this to focus on the Asian Championships in November and the Commonwealth Games next year.
While Ankush outpunched Mongolia's Dulgunn Oyunchimeg, Priyanka got the better of Russia's Aleksandra Ordina.
Ankush will be up against Russian Radna Tsibikov in his last-four stage. Priyanka, on the other hand, will square off against Korea's Hye Song Choe.
Hussamuddin (56kg) outpunched China's Ma Xin Ming, Shyam Kumar (49kg) got the better of Mongolia's Enkhmandakh Kharkhuu in his last-eight stage contest.
After today's results, India have one woman and four men in the semifinals.
Yesterday, Commonwealth Games silver-winner L Devendro Singh (52kg) assured himself of his first international medal by entering the last-four stage.
Devendro, who jumped to the flyweight category late last year, defeated Russia's Dimitrii Yusupov to advance in the tournament.
The triumph is a major confidence-booster for the 23- year-old from Manipur, who missed out on the Asian Championships earlier this year after surprisingly going down in the trials. He had also lost early in the Strandja Memorial in Bulgaria and the King's Cup in Thailand.
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