Investigators were looking into the cause of one of Europe's worst road accidents when a coach carrying 48 people plunged off a highway viaduct near Naples on Sunday.
Prime Minister Enrico Letta joined the throng of mourners at the funeral in Pozzuoli, a southern Italian industrial town where most of the victims came from.
"My brother was killed, it has broken me," said Fabrizio, a local resident in his forties who looked exhausted after a night's vigil at his brother's side.
Around a dozen people fainted during a funeral mass and were carried out on stretchers. Two women had fits and were removed screaming and thrashing.
"We feel terrible. We all know each other here. We are like brothers and sisters," said one tearful mourner, Franco, who lost a friend in the accident.
The names of the victims were read aloud before local Catholic bishop Gennaro Pascarella led the mass assisted by priests who comforted the relatives and gave communion.
"Political and religious institutions should not leave our brothers alone," he said, before the coffins were raised to applause and carried away.
Prosecutors are considering possible manslaughter charges over Sunday's accident and have said several people are already under investigation.
Hundreds of relatives yesterday had to spend agonising hours identifying the bodies of the victims close to the scene of the crash before the coffins were carried off.
The coach had rammed several cars after failing to brake on a bend, smashing through a highway crash barrier and plunging 30 metres (100 feet) down a slope.
One survivor said she thought the bus had lost a wheel before the crash and that the driver had tried to brake by sliding along the guardrail.
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