"Personally, I am not only convinced that a chemical attack has taken place ... I am also convinced that the Syrian regime is responsible," Rasmussen said when asked if he had seen intelligence to prove Assad was behind the suspected attack which left hundreds dead.
"In general, we have concrete information," he said.
Rasmussen stressed repeatedly that the international community could not allow the use of chemical weapons go by unchallenged but he was guarded about any military response.
Any military action, however, "would be very short, sharp, tailored," Rasmussen said, adding: "I don't see a long-term, sustainable military solution in Syria ... We need a political process."
It would be up to NATO's 28 members, led by the United States, to decide what specific military response they might make, he said.
"I don't envisage any further NATO role" beyond providing a forum for consultations and backing up member Turkey which shares a tense border with Syria, he added.
The Patriot missiles have a deterrent effect, Rasmussen said when asked about a possible change in NATO's stance if Syria were to retaliate against Turkey for a US strike.
"An attack on one ally is an attack on all," he said, recalling NATO's core rationale and mission.
"I can assure you we have all plans in place for an effective defence of Turkey ... I don't see any need for extra contingency planning."
Britain has backed out of any military commitment after a parliamentary vote last week blocked efforts by Prime Minister David Cameron to join US forces in military action, leaving France the only major European country still committed to a strike.
