The discovery is a reminder of just how little scientists still know about the natural world, even when it comes to such charismatic creatures. The findings also have important conservation implications for the cats, researchers said.
Scientists had thought that there was a single species of housecat-sized Brazilian tigrina.
However, the molecular data now show that tigrina populations in northeastern versus southern Brazil are completely separate, with no evidence of interbreeding between them. As such, they are best described as two distinct species.
"Our study highlights the need for urgent attention focused on the Brazilian northeastern tigrinas, which are virtually unknown with respect to most aspects of their biology," said Eduardo Eizirik of Pontificia Universidade Catolica do Rio Grande do Sul in Brazil.
That evolutionary history includes ancient hybridisation and movement of genes between the pampas cat and the northeastern tigrinas (Leopardus tigrinus).
In contrast, southern tigrinas (newly recognised as Leopardus guttulus) continue to hybridise with Geoffroy's cats, leading to extreme levels of interbreeding between the species along their contact zone.
Those patterns add to evidence that hybridisation can and does occur between distinct animal species.
As for the two tigrina species, the researchers suggest that they may be suited to different habitats, with the northeastern cats living primarily in savannahs, as well as dry shrub lands and forests, and the southern species living in denser and wetter Atlantic forests.
"All four species are threatened, and we need to understand as much as possible regarding their genetics, ecology, and evolution to be able to design adequate conservation strategies on their behalf," Eizirik added.
The study was reported in the Cell Press journal Current Biology.
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