The discussions with Chancellor Angela Merkel are expected to focus on the negotiations on a free trade agreement (FTA) between the US and the European Union and resolving the Syrian conflict.
The controversial US internet and telephone surveillance programme will also feature in the talks.
Obama flew into the German capital accompanied by his wife Michelle Obama and his two daughters after attending the G-8 summit in Northern Ireland for a day-long visit.
His speech has evoked memories of his predecessor, the late president John F Kennedy's historic "ich bin ein Berliner" address in the divided city exactly 50 years ago and two years after the building up of the Berlin Wall.
In his address, Obama is expected to reaffirm the United States' adherence to trans-Atlantic partnership and to allay fears that a recent shift in US foreign policy focus towards the Asia Pacific region will not be at the cost of its close ties with Europe.
The visit is taking place amidst some of the most comprehensive security arrangements ever mounted in the city for a foreign dignitary. The airspace over Berlin was closed for all flights shortly before the presidential jet Air Force One approached the city and the route his motorcade took from the airport to his hotel was kept a closely guarded secret.
Meanwhile, several organisations are planning protest demonstrations against Obama's failure to close the Guantanamo Bay detention centre, deployment of US combat drones in Afghanistan and Pakistan and US telephone and internet surveillance around the world.
Chancellor Angela Merkel, whom Obama met at the G-8 summit, will receive him shortly afterwards at the chancellery and afterwards they will hold discussions over a working lunch.