The video was released a day ahead of the RK Nagar by- poll in Chennai.
Ramadoss raised doubts over the timing of the release of the video.
"We don't know the authenticity of the video, whether it was of the hospital or her (Jayalalithaa) stay in her home. Why was it released a day before the poll... Inquiry should be done... They (TTV Dhinakaran faction) should be booked," the Pattali Makkal Katchi (PMK) leader said.
The one-man justice A Arumughaswamy commission is also looking into the medical treatment provided to Jayalalithaa.
The 20-second footage, aired by TV channels, showed the AIADMK supremo in a nightdress, looking frail and sipping what appeared to be a health drink with the help of a straw.
The ruling AIADMK said it was a "conspiracy" and a "demeaning" act with an eye on tomorrow's RK Nagar bypoll.
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