"An American government that chooses to trample on her legal and legitimate international commitments, a conversation with such a government would be a waste of time," Rouhani told reporters on the sidelines of the UN General Assembly.
Iranian Foreign Minister Mohammad Javad Zarif was set today to meet his US counterpart Rex Tillerson for the first time as part of the accord, which also involves five other major powers.
But he said there was no question of reopening negotiations, a day after Trump called the accord an "embarrassment."
"We were able to agree on mutually acceptable dates and deadlines so this agreement is not something you can touch. If you take out a single brick, the entire building will collapse," he said.
Rouhani said that Iran had "various options" including "the removal of obstacles" if the United States pulls out of the agreement.
"It means that our hand would be completely open to take any action that we see as beneficial to our country," he said.
He declined to be more specific but said that Iran will never seek nuclear weapons.
The dispute centers on uranium enrichment, which Iran says is for civilian use but critics led by Israel say is intended to build nuclear weapons.
Rouhani said that Iran would also need to look at the stance of the other parties to the agreement -- Britain, China, France, Germany and Russia -- if the United States pulls out.
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