The Kremlin's latest statement comes after a protester was killed in clashes in the east of the ex-Soviet country yesterday, and as Russian Foreign Minister Sergei Lavrov and US Secretary of State John Kerry hold talks on the Ukraine crisis in London.
Russian forces have occupied the Crimean peninsula and thousands of troops have staged drills near the country's eastern borders.
"Russia recognises its responsibility for the life of its compatriots and fellow citizens in Ukraine and reserves the right to protect people," the Russian foreign ministry said.
In the east Ukrainian city of Donetsk yesterday, a pro-Kiev protester was stabbed and killed and several were injured in clashes, marking the first death since tensions erupted in the southeast of the country after pro-Russian forces took control of Crimea.
"We have repeatedly stated that those who have come to power in Kiev should disarm militants, ensure the safety of the population and people's legal right to hold rallies," the Russian foreign ministry said.
Historic and linguistic faultlines divide the ex-Soviet nation of 46 million into the Russian-speaking, Moscow-leaning east and the Ukrainian-speaking, pro-European Union west.
Russia stunned the West by sending troops to the Crimean peninsula after a popular uprising in Kiev ousted pro-Moscow president Viktor Yanukovych last month.
Observers have warned that the raging crisis could ultimately split Ukraine.
On Sunday, Crimea is preparing to hold a referendum which is expected to cement Russia's takeover of the strategic peninsula, home to its Black Sea fleet.
Political analyst and Kremlin adviser Dmitry Orlov said he was not expecting Russia to launch a full-scale assault against Ukraine.
"This is a signal to aggressive groups in Ukraine who are seeking to destabilise the situation in the country," he said, referring to the statement.
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