All 12 were waitresses at a North Korea-themed restaurant in China who arrived in the South with their manager, making headlines as the largest group defection in years.
While Seoul says they fled voluntarily, Pyongyang claims they were kidnapped by South Korea's National Intelligence Service (NIS) and has waged a campaign through its state media for their immediate return.
The liberal lawyers' group, called the Lawyers for a Democratic Society, has been seeking direct access to the defectors to determine their version of events.
But the NIS said they were unwilling to testify and refused to bring them to court.
In the police complaint submitted today, the lawyers asked that NIS chief Lee Byung-Ho be investigated for abuse of power, accusing him of holding the women against their will and denying them access to the lawyers.
"Given the highly isolated circumstances around the defectors and their lack of knowledge in the South's legal system, it is very suspicious whether every one of them firmly agreed not to make an appearance at the court," the lawyers said in a statement.
Most new arrivals from the North are held for about three months at an NIS interrogation facility for screening for potential spies, before being sent to a resettlement centre for three months' training.
But the NIS announced this week that the 12 women would remain in its protective custody, rather than being sent to the centre.
The dispute over the defectors has fanned inter-Korean tensions that have been running high since the North's fourth nuclear tests in January.
But group defections are rare, especially by staff who work in the North Korea-themed restaurants overseas and who are handpicked from families considered "loyal" to the regime.
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