The second seeded Indo-American pair had recovered well after losing the first set and had chances in the match tie-breaker but eventually lost 3-6 6-3 9-11 in one hour and 20 minutes to the Romanian-Czech combination.
It was second match in a row where Sania and Huber faced stiff competition from their rivals.
They now play Wimbledon, where they have been seeded sixth.
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