Gregory Hartl, a spokesman for the organisation, confirmed that Saudi Arabia, which leads an Arab coalition battling rebels in Yemen, had signed an agreement committing the funds to help the WHO battle the spread of cholera.
The oil-rich Gulf kingdom, in a statement on Thursday, said the money committed to WHO was part of an overall effort to combat the cholera outbreak in Yemen.
Riyadh said it also pledged USD 33 million to the UN's children agency, UNICEF, for a project to improve water and sanitation facilities which are "drivers of the epidemic".
Last week the ruler of Dubai, Sheikh Mohammed bin Rashid al-Maktoum, also pledged USD 10 million to help the WHO stem the spread of cholera in Yemen.
The United Arab Emirates is a key member of the Saudi-led coalition that entered Yemen's conflict in 2015 on the side of the government against Iran-backed Shiite Huthi rebels.
Since then, the war in the impoverished Arabian Peninsula nation has killed more than 8,000 people, according to WHO figures.
The cholera outbreak has already claimed the lives of at least 1,915 people since April this year, with 436,000 suspected cases across the country.
It also warned that the number of cholera cases was expected to reach 600,000 by the end of the year.
The report was issued after executive directors of WHO, UNICEF and the World Food Programme visited Yemen to assess the situation.
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