The 30-share index, which had gained 48.61 points yesterday, fell by 75.07 points, or 0.40 per cent, to 18,683.56, with stocks of oil and gas, IT and banking sectors succumbing to profit-booking.
Similarly, the wide-based National Stock Exchange Nifty Index moved down by 23.20 points, or 0.40 per cent, to 5,682.10.
Brokers said apart from profit-booking by investors after yesterday's gains, a weak trend in the Asian region ahead of US economic growth figures to be released later in the day, mainly led to a fall in stock prices.
Meanwhile in the Asian region, Japan's Nikkei was down by 0.89 per cent, while Hong Kong's Hang Seng index shed 0.79 per cent.
The US Dow Jones Industrial Average ended 0.20 per cent higher in yesterday's trade.