Ten trawlers of the fishermen were also seized by the Sri Lanka Navy yesterday.
Sri Lankan Navy spokesman Kosala Warnakulasuriya said 49 fishermen had been arrested on two separate occasions off the northern coastal areas of Kankesanturai and Talaimannar.
While 24 of them were handed over to the Talaimannar police yesterday the rest of them were to be handed over to the Kankesanturai police today.
They will be produced in court and charged for violating Sri Lanka's international maritime border line, the Navy said.
