Details on how she is returning are being kept under wraps, reported Entertainment Weekly.
A series regular, Thompson, 58, left The CW show after season 2 when the Queen matriarch was killed by Deathstroke.
Even Deathstroke will return in the 100th episode, as revealed in a photo posted by Stephen Amell.
Katie Cassidy and Jamey Sheridan will also reprise their roles as Laurel Lance and Robert Queen, respectively, in the episode.
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