The ceasefire deal, announced Friday after marathon talks between Russia and the United States, has been billed as the best chance yet to halt the bloodshed in Syria's five-year civil war.
As well as bringing a temporary halt to the fighting, it aims to provide crucial aid to thousands of desperate civilians, many of whom suffered an especially bloody weekend of air strikes ahead of Monday's deadline.
Under the deal, an initial 48-hour ceasefire is to begin at 7:00 pm local time in Syria, halting fighting in areas not held by jihadists like the Islamic State group.
If the ceasefire then holds for a week, Moscow and Washington are to begin unprecedented joint targeting of jihadist forces including IS and the former Al-Qaeda affiliate Fateh al-Sham Front.
After years of stalled peace efforts and the failure of a landmark truce agreed in February, world powers are anxious to bring an end to a conflict that has left more than 290,000 dead and forced millions from their homes.
"We want to know what the guarantees are," Salem al-Muslet, a spokesman for the High Negotiations Committee, the main opposition umbrella group, told AFP.
"We are asking for guarantees especially from the United States, which is a party to the agreement."
He said key questions included how the deal would define "terrorist" groups that can continue to be targeted and what the response would be to violations of the truce.
"We fear that Russia will classify all the Free Syrian Army (rebel factions) as terrorists," he said.
Questions also remain about how the ceasefire will apply in several parts of the country where the Fateh al-Sham Front, previously known as Al-Nusra Front, is present.
The group cooperates closely with many of Syria's rebel forces, including moderate rebels and the powerful Ahrar al-Sham faction, which on Sunday issued a scathing condemnation of the Russian-US deal.
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