The Uttar Pradesh first innings lasted 74.4 overs at the MA Chidambaram Stadium.
At stumps, left-handed opening batsman Ahbinav Mukund (26) and Baba Aprajith (1) were at the crease.
Earlier, electing to bat, Uttar Pradesh opening batsman Tanmay Srivastava offered a straightforward catch to medium pacer Vijay Shankar in the fourth over of the day.
With the other opener Akash Varma (16) also back in the pavilion after being caught at short leg by Baba Indrajit off M Prabhu's bowling, the visitors were struggling at 36 for two, before slipping to 89 for five in the 40th over.
In Tamil Nadu's innings, spinner Piyush Chawla who came on to bowl in the eighth over, dismissed opening batsmen Umasharnkar Sushil.
Mukund and Shankar (17) seemed to be steadying the innings, but the latter stepped out against Harish Alam and was stumped by wicketkeeper Ekalavya Dwivedi. The two had put on 47 runs in 14.2 overs for the second wicket.
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