Scores of shuttle buses take government and military personnel to work every morning in the capital, and they have often been targeted by insurgents despite efforts by security forces to provide better protection.
Yesterday, Taliban suicide bombers killed at least seven people in twin attacks on two buses.
The militant group, which claimed responsibility for all three blasts, strongly oppose the US troop agreement that was signed on Tuesday.
"The wounded were immediately taken to the hospital, and they are in a stable condition."
A police statement confirmed the death toll and injured.
Taliban spokesman Zabihullah Mujahid said in a text message: "Around 7:00 am, a suicide attacker targeted a bus carrying Afghan security forces, as a result, a number of officers were killed and wounded."
Afghanistan and the United States on Tuesday signed the long-delayed bilateral security agreement (BSA) to allow about 10,000 US troops to stay in the country next year.
After yesterday's blasts, the Taliban said they were sending "a clear message to the stooge government that signed the slave pact, and we will step up our attacks after this".
The BSA was signed by Afghanistan's new "national unity government", which was formed under a US-brokered agreement to solve months of disputes over who won the fraud-tainted presidential election.
Ghani, who was declared the election winner, agreed to a power-sharing deal with his poll rival Abdullah Abdullah, who has taken up the new role of chief executive.
It dismissed the elections as "a publicity stunt" and said that the US had "lost all possibilities to win" the war, but made no direct mention of the BSA or the new government.
Hamid Karzai, who stood down as president on Monday, had refused to sign the BSA, underlining rocky Afghan-US relations over recent years.
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