On the eve of the vote, the Palaniswami camp today got a jolt when one of its MLAs R Natraj, a former state DGP, said he would vote against the chief minister's motion of confidence.
Natraj's move has reduced Palaniswami's stated support to 123 members in a House of 234.
The floor test in the Assembly will be the first such exercise the state will witness in about 30 years.
"I have interacted with people in my constituency and their considered and overwhelming opinion is that the government of O Panneerselvam should continue and I have to reflect this opinion of the people in the Assembly," Natraj, a Mylapore MLA, told PTI.
To a question, Nataraj said he does not consider this as a confidence vote, but a 'conscience vote.'
Before Natraj announced his decision, Palaniswami has claimed the support of 124 MLAs and asserted his government will stay while his rival and predecessor O Panneerselvam has vowed to continue his fight against Sasikala and her family "till the time Amma (Jayalalithaa) regime is restored."
AIADMK has 134 MLAs in the 234-member legislative assembly.
Palaniswami will be seeking the confidence vote in line with Governor Ch Vidyasagar Rao asking him to do so yesterday while inviting him to form the government.
Though the new government was given 15 days time to prove the majority in the House, Palaniswami, a known loyalist of AIADMK general secretary V K Sasikala, opted to do it within two days.
A revolt by Panneerselvam against Sasikala last week resulted in a political stalemate with AIADMK supporters divided between the two camps.
In an intra-party power struggle, Panneerselvam had rebelled against Sasikala on February 7, alleging he was forced to step down to make way for her becoming Chief Minister.
She had been elected the AIADMK Legislature Party leader on February 5.
Incidentally, it was a succession row that triggered a faction war in AIADMK that had prompted a floor test in the Tamil Nadu Assembly in January 1988, when Janaki, widow of M G Ramachandran, succeeded him in the midst of a bitter rivalry involving Jayalalithaa.
Amid chaotic scenes and a spate of disqualifications by then Speaker P H Pandian and boycott by the opposition, Janaki had managed to win the confidence vote.
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