Ashmeade, part of the gold medal-winning Jamaican 4x100m relay team from the Moscow worlds last year, clocked an easy-going 10.40 seconds to win his heat at a packed Hampden Park, home to the Scotland international football side.
The weight of the Caribbean island's expectations are resting squarely on Ashmeade's shoulders in the absence of sprint superstar Usain Bolt, who will compete solely in the relay in Glasgow after an injury-plagued season.
"I'm loving it, I'm very lucky to come to a place like this, the reception was amazing," Gemili said.
"I think I'm going to have to break 10 seconds to get a gold medal, these guys are running really fast."
Trinidad and Tobago's Thompson, the 2008 Olympic silver medallist, could only finish third in his heat.
But his time of 10.33sec behind US-born Nigerian Mark Jelks, who has served a two-year doping ban, and Warren Fraser of the Bahamas saw him qualify for Monday's semi-finals as one of the six fastest outside the top two in each of the nine heats.
In the women's 100m heats, it also wasn't all plain sailing for Thompson's teammate Michelle-Lee Ahye, who rebounded from a sluggish start to come in second in her heat in 11.44 behind Canadian winner Khamica Bingham.
Jamaica's Veronica Campbell-Brown looked slick in winning in 11.29sec and she will be joined in the semi-finals by teammates Schillonie Calvert, who clocked the same time in nailing her heat, and Olympic silver medalist Kerron Stewart (11.35).
It was Nigerian Blessing Okagbre, who won long jump silver and 200m bronze at the 2013 world championships, who headed the qualifying times with 11.20sec.
