The blast happened in the area of Sarikamis on the outskirts of the majority Kurdish city "while PKK militants loaded explosives onto a stolen truck", the ministry said, referring to the Kurdistan Workers Party.
"The explosives detonated prematurely. The injured were civilians, according to initial findings," the ministry said yesterday.
An AFP correspondent in Diyarbakir said the impact of the blast was felt in several neighbourhoods of the city.
The explosion came hours after at least eight people including soldiers were injured by a remotely-detonated car bomb aimed at a military vehicle in Istanbul, according to the local governor's office.
Fighting between Turkish security forces and the PKK, which is listed as a terrorist organisation by Ankara and its Western allies, erupted last summer after the collapse of a two-year ceasefire.
Turkey is on edge after two deadly attacks in Istanbul this year blamed on Islamic State (IS) jihadists, and a pair of attacks in Ankara that were claimed by Kurdish militants and killed dozens.
Three people were killed Tuesday and 42 others wounded when a car bomb blamed on PKK militants struck a police vehicle in Diyarbakir.
The two attacks in Ankara were claimed by the Kurdistan Freedom Falcons (TAK) - a radical splinter group of the better-known PKK.
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