Acting President Oleksandr Turchynov told the parliament in Kiev that rebels used a portable air defence missile to bring down the helicopter. He said 14 died, including Gen Serhiy Kulchytskiy, according to the Interfax news agency, which earlier gave the wrong first name for the general.
Ukraine's National Guards put the death toll at 12, including Kulchytskiy, and said that one soldier was badly wounded, but added that the information is still being clarified.
An Associated Press reporter saw the helicopter go down amid a trail of black smoke. Gunshots were heard in Slovyansk near the crash site and a Ukrainian air force jet was seen circling above. It was too dangerous to visit the site itself.
Turchynov said the helicopter was flying troops to a hill outside Slovyansk where Ukrainian forces have set up positions.
Slovyansk is in the Donetsk region, one of the two provinces in eastern Ukraine that have declared independence from the government in Kiev.
The Kiev government condemns the insurgency roiling the east as the work of "terrorists" bent on destroying the country and accuses Russia of fomenting it. Russia denies the accusations, saying it has no influence over rebels, who insist they are only protecting the interests of Russian-speakers in the east.
Still, fighters from Russia, including from the battle-hardened region of Chechnya, have been appearing recently in the ranks of the separatists.
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