Mark Lippert, 42, underwent surgery to treat deep gashes to his cheek and hand after he was slashed by a knife-wielding nationalist at a breakfast function in central Seoul last Thursday.
Hospital officials said that after five days of treatment the US diplomat had now had all 80 stitches from his face removed and the pain in his left wrist had subsided.
"It was obviously a scary incident but I am walking, talking, holding my baby, hugging my wife. So I just feel really good", the new father said, adding he would get back to work "as soon as possible".
Lippert, whose popularity among South Koreans has only grown since the attack, expressed gratitude at the "outpouring of support" he and his family have received.
"We have made it our mission to be open and friendly. And that will not change," he said.
The assailant Kim Ki-Jong was formally charged with attempted murder on Friday, despite his pleas that he never intended to kill the ambassador.
Asked if he had carried out the assault on the orders of North Korea, Kim insisted he acted alone, while North Korea accused the South of launching a "vicious" smear campaign to tie its leadership to the attack.
Kim, 55, has a previous conviction for hurling a rock at the then-Japanese ambassador in 2010.
Kim has said he attacked Lippert as a protest against ongoing South-US army drills, which he blamed for souring inter-Korean relations.
The annual drills, known as Foal Eagle and Key Resolve, have long been condemned by Pyongyang as rehearsals for invasion.
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