Since 1995, the US military has used the Russian-made engines on Atlas V rockets, which are often employed to send Air Force and other satellites into space.
"In light of the current situation, the department has directed the Air Force to perform an additional review to ensure we completely understand the implications, including supply interruptions, of using the Russian-made RD-180 rocket motor in our Atlas V launch vehicle..." spokeswoman Maureen Schumann said.
After earlier reviews, officials stockpiled a supply of the Russian rocket engines and now have an inventory that would last about two years, according to Schumann.
The latest US sanctions imposed against Russia over its intervention in Ukraine's Crimean peninsula do not effect any Pentagon programs, she said.
If the Atlas V with its Russian engines were scrapped, it would take up to five years and as much as a billion dollars to replace, she said.
Schumann called the Atlas V a "reliable, cost-effective launch vehicle with a proven record of success," and alternative launch vehicles for larger satellites would carry a higher price tag.
A senior US defense official, speaking on condition of anonymity, told AFP that it was unlikely the review would lead to any major change in the launch program or a decision to drop the Russian engines.
Separately, lawmakers in Congress have renewed demands for the Pentagon to cancel a contract for Russian-made helicopters for the Afghan government, citing Moscow's actions in Ukraine.
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