State Department spokeswoman Heather Nauert told reporters that US Ambassador Nikki Haley would oppose the measure when Cuba brings it before the General Assembly on Wednesday.
"We've historically voted against that resolution. Last year, the United States abstained," Nauert said, looking back on a 26-year ritual that has seen Washington isolated on the issue.
"Ambassador Haley will be reversing last year's abstention and will be voting against the resolution this year," she said.
"The Trump policy on Cuba gives greater emphasis to advancing human rights and democracy in Cuba while maintaining engagement that serves US national interests," she said.
Trump's predecessor, former president Barack Obama, oversaw slowly warming ties with Havana and a loosening of the trade embargo, symbolised by last year's decision to abstain.
But Trump has tightened up some of the openings that had begun to appear in the embargo and has hardened the tone against the communist-run island, casting a new chill on ties.
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