Born on March 26, 1986 when US President Ronald Reagan was in the White House, Scooter had been undergoing medical testing and treatment after breaking his leg in October 2014.
"He was so special. I don't think I'm ever going to get over this," said his owner Gail Floyd from Mansfield, Texas.
Floyd said Scooter, who had travelled with her to 48 of the 50 US states, died by the time Guinness had conferred its title on April 8.
"He likes to see new places and he likes people," Floyd, said recently while describing Scooter as "an active cat, full of energy and playfulness".
"Scooter is quite an amazing cat, with a strong will to live," the cat's veterinarian was quoted as saying by the 'Daily Express'.
As a playful kitten, Scooter liked nothing better than playing with his owner's hair and riding on her shoulders.
He had taken the world's oldest cat title from a 26-year-old American tabby called Corduroy, who lives in Oregon.
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