Following Granny and other matriarch killer whales has shown their crucial role within the family group.
They guide the pod as it forages, take care of other females' young calves and even feed the larger males, researchers said.
These post-reproductive female leaders help their families to survive, and the advantage they offer could show what drives a species to evolve to stop reproducing.
"It was inevitable that this day was going to come but it is very sad news and a further blow to this population," said Darren Croft from the University of Exeter in the UK, who leads this evolutionary biology research.
The orcas of an area known as the Salish Sea - close to Vancouver and Seattle - have been the subject of a four decades long study led by Ken Balcomb from the Center for Whale Research (CWR). He had first photographed Granny, official named J2, in 1976, 'BBC News' reported.
On the centre's website, which first reported Granny's death, Balcomb wrote that he last saw her on October 12 last year as she swam north far ahead of the others.
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