"We have still not found any involvement (of ISIS). We have to investigate," Hasina told reporters at Ganabhaban, her official residence in the capital.
Describing the murders of a Japanese and an Italian as "clearly planned and politically motivated", she said the killings were part of a conspiracy to "tarnish the image" of her government.
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The BNP-Jamaat have "definitely abetted these murders in an attempt to overshadow Bangladesh's achievements", she said.
Hasina said that the murder of foreigners have taken place at a time when the war crimes trials are taking place.
The war crimes trials involve top Jamaat leaders who were opposed to Bangladesh independence from Pakistan in 1971. Some of the Jamat leaders have already been sentenced to death.
"We will take action and track down the culprits. But if we get carried away by these murders and overlook our achievements, the BNP-Jamaat conspiracy will be successful," Hasina said.
Japanese national Hosi Koniyo, 66, who was headed to an agriculture farm on the outskirts of northern Rangpur city in a rickshaw, was shot thrice in the chest, shoulder and hand by the unidentified assassins yesterday. He died instantly.
In a statement in Arabic language, the ISIS claimed responsibility for the attack on Koniyo, according to SITE Intelligence Group, which monitors jihadi postings online.
Koniyo's murder came five days after 50-year-old Italian aid worker Cesare Tavella was killed when motorbike-born terrorists shot him dead in Dhaka's diplomatic area.
The ISIS had also claimed responsibility for the killing of the Italian aid worker last week in Bangladesh's capital.
"We've got no clues. If someone claims responsibility, why should we have to accept it?" Hasina said.
"Until we find out the link through investigation, I don't think there is any reason for us to accept it," she said, adding that intelligence agencies are working on that.
Koniyo had been in Rangpur for the past six months and was familiar in the neighbourhood because of his philanthropic work. He had set up the farm, where he visited daily, with help of a local businessman to grow grass for cattle.
Police detained the businessman, along with the rickshaw puller, Koniyo's landlord and another resident of the area, for questioning.
Bangladesh had previously downplayed the ISIS claim of killing of the Italian worker, describing it as an "isolated incident".
Security officials, either, have not ruled out that the ISIS could be using "local sympathisers" to carry out such attacks.
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