Sisters Serena and Venus Williams, who have won the doubles title five times, withdrew from the Wimbledon women's doubles on Tuesday.
No immediate reason has been given for the duo's withdrawal till now.
Both sisters are still involved in the singles tournament and there is a huge possibility that they could meet in the fourth round.
Their place in the draw was taken by lucky losers Misaki Doi of Japan and Switzerland's Stephanie Vogt, Sport24 reported.
Venus said, "We're looking forward to Rio 2016. We are trying to get an early start on preparing, start playing some doubles matches, get the hang of it."
The sisters, who have won 13 doubles majors together, had earlier withdrawn from the Australian Open as well.
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