The blast struck the mosque in the village of Kouyape, in Kolofata district north near the Nigerian border, at around 5:30 AM (1000 IST) during morning prayers, a security source said.
Since July last year Cameroon's far north has been hit by a series of attacks blamed on Boko Haram, which has pledged allegiance to the Islamic State group.
"Eleven worshippers were killed at the scene. A twelfth died of their wounds in hospital," the security source said, adding that the attacker was praying alongside other worshippers when he blew himself up.
The bombing came after two people were killed overnight in the same area in another attack blamed on Boko Haram, the security source said.
Cameroon has beefed up its military presence along the Nigerian border as part of a regional coalition, after years of doing little to stop Boko Haram fighters using its territory as a rear base to arm and equip themselves.
Since late November the Cameroon army has carried out operations in several border areas aimed at weakening Nigerian jihadists who have been very active in the region.
Over the past year, Boko Haram has stepped up cross-border attacks in Niger, Chad and Cameroon while also continuing to mount shooting and suicide assaults on markets, mosques and other mostly civilian targets within Nigeria itself.
The group's six-year campaign for a hardline Islamic state in northeast Nigeria has killed at least 17,000 people and made more than 2.6 million others homeless.
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