The explosion happened at the Tashar Dan-Borno motor park on the outskirts of the city immediately after a man put a bag in the boot of the bus and then tried to board.
"The bus had just loaded with passengers on its way to Kano when a huge explosion happened inside the bus at exactly 11:40 am (1040 GMT)," said a driver's union official at the bus station.
Thirteen dead and 31 injured were initially brought to Potiskum General Hospital after today's blast, according to a nurse at the facility, but she added: "Four more died here.
"So, we have 17 dead and 27 people with injuries."
The drivers' union official said it was not immediately clear whether the man who placed the bag in the boot was a suicide bomber or whether the explosives were hidden inside it.
The girl in Sunday's attack which left seven people dead in Potiskum was thought to be as young as seven, according to multiple witnesses.
Boko Haram Islamists have increasingly used young girls and women as human bombs, with so-called "soft targets" such as markets and bus stations hit regularly.
Both bombings again underlined the severe security challenges facing Nigeria in the run-up to presidential and parliamentary elections on March 28.
The elections were initially scheduled for February 14 but were delayed by six weeks to give the military and its allies more time to secure and stabilise the northeast to allow people to vote.
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