The attack came shortly after Israeli police said they shot dead a Palestinian who tried to stab police officers in annexed east Jerusalem.
"Two assailants rammed a vehicle into a civilian bus stop at the Elias junction near the community of Kiryat Arba," the Israeli military said in a statement.
It said three people were wounded.
"Forces at the scene fired at the vehicle resulting in the death of one of the assailants while the other was wounded," the army said.
Kiryat Arba is an Israeli settlement in the southern West Bank close to the flashpoint Palestinian city of Hebron.
It was the second violent incident today, after a 28-year-old man was shot dead in Jerusalem while attempting to stab police officers.
That attack came at the Damascus Gate entrance to east Jerusalem's tourist-heavy Old City, the main entrance for Palestinians.
Violence since October has killed 227 Palestinians, 34 Israelis, two Americans, one Eritrean and a Sudanese.
Israeli forces say most of the Palestinians killed were carrying out knife, gun or car-ramming attacks. Others were shot dead during protests and clashes.
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