"The death toll is now 36," said Rift Valley traffic police chief Zero Arome, explaining the initial toll of 30 had risen, "after six passengers succumbed to injuries in hospital."
The accident occurred at 3:00 am (0000 GMT) close to a notorious stretch on the Nakuru-Eldoret highway.
A bus travelling from Busia, in western Kenya, collided with a truck coming from Nakuru town.
Police said the death toll for that stretch of road has now topped 100 this month alone.
One survivor, speaking from his hospital bed, said he had been asleep at the back of the bus when the collision happened.
"All I heard was a loud bang and screams from all over," he said. "I was seated at the back and was helped out after some time because my legs were stuck. It is by the grace of God that I am alive. I saw many people dead and their bodies mutilated."
In December last year more than 40 people died when an out of control fuel tanker ploughed into vehicles and then exploded on another busy stretch of highway. Deaths from road accidents commonly spike during the holiday period when people criss-cross the country visiting relatives.
In recent weeks road accidents have claimed the lives of hundreds of people, among them three Pentecostal bishops and a newly-elected governor.
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