Forbes magazine said its real-time tracking of personal fortunes showed Bezos with a net worth of USD 90.5 billion, ahead of the USD 90 billion for Gates early today.
A few hours later, though, Bezos had slipped back to second place as Amazon shares pared their gains.
Bezos owns around 17 per cent of the equity of Amazon, which has been expanding from its original mission as an online retailer to a diversified tech firm in cloud computing, online video, computer hardware and artificial intelligence.
Amazon shares were up 1.7 per cent at USD 1,070.72 and have risen some 24 per cent over the past four months, adding some USD 17 billion to the net worth of the 53-year-old Bezos.
According to Forbes, Gates has been the richest person in the magazine's annual rankings in March for the past four years and for 18 of the past 22 years. Mexican telecom magnate Carlos Slim overtook Gates from 2010 to 2013.
Among the billionaires gaining ground is Facebook founder Mark Zuckerberg, who has an estimated worth of some USD 71.3 billion according to the real-time Forbes estimate.
Forbes pointed out that Gates could have been considerably wealthier, having given away more than USD 30 billion to philanthropic causes over the years.
Bezos has given an estimated USD 100 million, but recently asked users on Twitter for ideas for charitable donations which could have an impact on the world.
The billionaire notably opposed Donald Trump during the 2016 presidential campaign, but after the election appeared to give the new president a boost by announcing Amazon would add 1,00,000 new US jobs over 18 months.
"So many stories about me in the @washingtonpost are Fake News," Trump tweeted this week.
"Is Fake News Washington Post being used as a lobbyist weapon against Congress to keep Politicians from looking into Amazon no-tax monopoly?"
Bezos appeared intent on spending more time in the US capital, purchasing what is likely the city's largest private residence for USD 23 million.
Under Bezos's ownership, the Washington Post reported it was profitable in 2016, after making a series of investments in new technology and personnel.
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