The app called Brush DJ was launched at the end of 2011 and in 2013 it was accepted into the UK National Health Service (NHS) Choices Health Apps Library.
By February 2015 Brush DJ, which is free with no advertisements or in-app purchases, had been downloaded on more than 197,000 devices in 188 countries. It can be used with any type of toothbrush, its makers said.
The app plays music for two minutes - the optimum time for brushing teeth - taken from a playlist or randomly from the user's own device or cloud.
A research on the app showed that 70 per cent of respondents reported their teeth felt cleaner since using the app and 88 per cent said that Brush DJ had motivated them to brush their teeth for longer.
Ninety per cent said they would recommend the app to their friends and family, researchers said.
"Brush DJ showed positive effect across four main themes - motivation, education, compliance and perceived benefits," Ben Underwood, dentist, app developer, NHS Innovation Accelerator Fellow and Honorary University Fellow at Plymouth University Peninsula Schools of Medicine and Dentistry, said.
"The results of our study indicate that apps such as Brush DJ are beneficial to users and open the way for further research to extend their use and effectiveness still further," said Underwood, who led the study.
The research was published in the British Dental Journal.
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