The activists' "Defend Europe" mission has been financed by a crowd-funding initiative organised by young anti- immigration campaigners from France, Italy and Germany.
Their 40-metre (130-foot) ship named "C-Star", hired by "Generation Identity", arrived on Saturday in an area where tens of thousands of migrants have been rescued from unseaworthy trafficker boats over recent months and years.
The boat spent 30-45 minutes tracking the Aquarius at a distance of a few hundred metres, before continuing to follow it from further back.
Its crew would not comment on whether they regarded the C-Star's proximity as intimidatory. Maritime charts indicated the NGO boat's speed had doubled in the time the far-right vessel was close to it.
The two boats were about 20 nautical miles off Libya in an area east of the capital Tripoli.
On its website, the Defend Europe alliance accuses NGOs of "smuggling hundred of thousands of illegal migrants to Europe, endangering the security and future of our continent" and vows to "do something against it."
The Defend Europe initiative has been denounced by humanitarian organisations as a potentially highly dangerous publicity stunt.
Forcing a migrant boat that had reached international waters back to Libya, where most depart from, would be illegal under international law.
NGO-chartered boats have rescued over a third of the nearly 100,000 people who have been picked up from often distressed trafficker vessels off Libya this year and taken to Italy.
Critics say the NGOs are making it too easy for the traffickers to guarantee would-be migrants safe passage to Europe, creating a "pull" factor at best and operating a taxi service at worst.
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