The navy yesterday shifted its mission from rescue to recovery, spokesman Enrique Balbi told reporters.
He said while it was not possible to definitively confirm the crew had perished, "no evidence of a shipwreck was found in the areas explored" and the international rescue effort had continued for "twice the time" it was estimated they could have survived.
"Despite the vast efforts made, we have been unable to locate the submarine," he said, though adding that international efforts to find the vessel would continue.
"We are in the middle of a tragedy. The atmosphere in the navy is absolute consternation, anguish and pain. We lost 44 comrades," said the officer, who spoke on condition of anonymity.
In addition to the loss of life, "half of Argentina's submarine capacity was lost," the officer said.
The country now has one active sub, while a second is undergoing repairs.
The US navy said yesterday it had deployed an unmanned recovery vehicle to join the hunt. The US had already provided other assistance, as did about a dozen other countries.
In its final communication, the submarine reported it had overcome a mechanical breakdown that resulted from a short circuit due to the entry of water via the vessel's snorkel.
Three hours later, a noise similar to an explosion was recorded 48 km from where the crew had given its last report.
The position was in line with the path the submarine would have taken to reach its base in Mar del Plata as planned, the navy has said.
Relatives of the missing had mixed feelings about yesterday's announcement.
"They just threw away the last hope we had," said Luis Tagliapietra, the 46-year-old father of one of the missing submariners.
But Jorge Villareal, whose son Fernando was the ship's lieutenant, refused to give up.
"I continue to maintain my hope and faith. Our optimism is intact, no matter what they say," Villareal told AFP.
Among the ARA San Juan's crew was Argentina's first female navy submariner: Eliana Krawczyk, 35.
And the navy has come under fire for its handling of the tragedy, as it failed to mention the problem reported by the vessel for several days after its disappearance.
Argentine President Mauricio Macri last week ordered an inquiry into the missing submarine, saying it requires "a serious, in-depth investigation that will yield certainty about what has happened.
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