The attack, for which there was no immediate claim of responsibility, targeted a checkpoint manned by forces of Libya's unrecognised government at Msillata, near Khoms, 120 kilometres from Tripoli.
"The car bomb explosion left six dead and 14 wounded, all civilians," Safwan Bayou, commander of a unit in charge of security on the coastal road linking eastern and western Libya, said.
An AFP photographer at the scene said the blast riped open a crater four metres (yards) across and three metres deep, destroying some 20 civilian and military vehicles.
Libya has had two administrations since August 2014, when a militia alliance overran the capital and set up its own parliament in Tripoli while forcing an internationally recognised government to take refuge in Tobruk, in the east.
The oil-rich north African country has been riven by chaos and warfare since the fall of longtime leader Moamer Kadhafi in 2011.
The United Nations has been brokering talks to create a new unity government to end fighting between the army and the militias that seized Tripoli.
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