The demonstrators were trying to reach Pearl Square, the focus of Shiite-led protests in 2011, after the funeral of a Shiite detainee who died in hospital in the capital on Friday.
The witnesses said several protesters were injured when the security forces intervened, although they were unable to give a specific number.
Demonstrators chanted slogans against the authorities in the Sunni-ruled kingdom, which has a Shiite majority.
Pearl Square was the epicentre of the 2011 Arab-Spring-inspired protests against the ruling Al-Khalifa dynasty.
In August, King Hamad decreed stiffer penalties for "terror acts".
These include a minimum 10-year jail term for an attempted bombing. If such attacks cause casualties, the sentence can be life imprisonment or the death penalty.
At least 89 people have been killed since the protests began two-and-a-half years ago, according to the International Federation for Human Rights.
Strategically located across the Gulf from Shiite Iran, Bahrain is home to the US Navy's Fifth Fleet and is an offshore financial and services centre for its oil-rich Arab neighbours.
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