The legal development comes as officials today said that the death toll from the country's worst industrial disaster had reached 645.
Sheuli Akter, wife of Jahangir Alam, filed the complaint with Dhaka magistrate Wasim Sheikh, saying her husband and other workers were "pushed toward death" by building owner Mohammed Sohel Rana and two others.
Alam was employed in New Wave Styles Ltd, one of the five garment factories housed in the eight-story Rana Plaza that collapsed April 24 as workers started their morning shift even though cracks had developed in the building.
A conviction for murder can result in a death sentence in Bangladesh. Nine people, including Rana and Adanan, have already been arrested on other charges. Rana faces charges such as negligence and illegal construction, which are punishable by a maximum of seven years in jail.
By afternoon today, the death toll had reached 645, according to the police control room at the scene. It is not known how many people are still missing, as workers use heavy equipment to search through the rubble. There is still a stench around the collapse site from decomposing bodies.
Officials say Rana illegally added three floors and allowed the garment factories to install generators.
Vibrations from garment machines and from the generators were thought to have contributed to the collapse.
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