Bolt and Gatlin are poised for the final chapter of their long rivalry here tomorrow when they are expected to duel once more for the title of fastest man on the planet.
The two sprinters easily negotiated their opening heats on a raucous morning session at the Olympic Stadium, with Gatlin topping the qualifying times with a tidy 10.01sec.
Jamaican superstar Bolt, bidding to win 100m, 200m and 4x100m titles for a third straight Games, was fourth quickest in 10.07sec.
"I'm feeling good. I'm happy. As I said, I've got the first one out of the way so I'm happy about that," Bolt said, predicting a fast semi-final.
"It's definitely going to be a good semifinal. There are a lot of guys running fast. It's good. It gets you running and gets you up to speed to go out there in the finals."
Gatlin, one of the oldest men in the field at 34, said he was kept on his toes by a crop of young sprinters.
Gatlin, who has twice been convicted of doping offences, has faced criticism from even within his own team at these Olympics, with teenage swimming gold medallist Lilly King saying last week athletes like him should not be allowed to compete.
But Gatlin bristled when asked to respond to the young swimmer's remarks following his race.
"I don't even know who Lilly King is -- she does swimming, not track and field. I'm not worried about that," Gatlin said.
The morning's only gold medal went to Germany's Harting, who saved his best for last to win gold.
It was an emotional win for Harting, whose famous shirt-ripping brother Robert -- the 2012 gold medallist -- failed to qualify for the discus final in Friday's preliminary rounds after suffering a back strain.
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