Cameron and other British political leaders headed to Scotland to rally voters to keep the 307-year-old union between England and Scotland intact and pleaded them to vote against breaking the UK in September 18 referendum.
"I love my country more than I love my party," an emotional Cameron said, campaigning in Edinburgh.
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"If Scotland votes for separation that would have to be respected by the rest of the United Kingdom. And as Prime Minister of the UK I would have to make that happen.
"It would be a heartbreaking job to have to do, to break up this family of nations that has been such a success," he added.
Cameron was in Scotland on the same day as Labour leader Ed Miliband and Deputy Prime Minister Nick Clegg make their own pleas for Scotland to vote 'No' to independence.
The Prime Minister's urgent trip follows a weekend poll putting the "Yes" camp two points ahead, and another yesterday showing both sides evenly split on 41%.
The close race ahead of the vote has sent shudders through the financial markets. Amid uncertainty over what currency an independent Scotland would use, the pound and shares in Scotland-based financial institutions have sinked.
He said Britain is "holding its breath" ahead of the vote.
"I don't want my children to grow up in a world where if they choose Edinburgh University they are going to be in a capital of foreign country," Cameron said.
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