Islamist supporters of deposed president Mohamed Morsi had already called for a boycott of the January 14-15 vote on a new constitution drawn up by the interim authorities since his July overthrow by the army.
But the Egyptian-born Qaradawi, who wields huge influence through his regular appearances on Al-Jazeera television from his base in exile in Qatar, said that participation would go against Islam because it would mean colluding with a "sin".
"Participation in the constitutional referendum -- and contributing in any way to strengthening this coup authority, or giving it legitimacy, or prolonging its existence and strengthening its presence -- is cooperating in a sin and an act religiously forbidden," the cleric said in the fatwa issued late yesterday.
He briefly returned to his homeland in February 2011, days after the overthrow of veteran strongman Hosni Mubarak, and delivered a sermon in Cairo's Tahrir Square, epicentre of the uprising.
Qaradawi's fatwa came as Morsi was back in court in Cairo today for a new hearing in his trial on charges of inciting the killings of opposition activists in clashes outside the presidential palace in December 2012.
The cleric is himself wanted by Egypt's interim authorities and faces trial in absentia.
The gas-rich Gulf state has given refuge to a number of Brotherhood leaders who fled the bloody crackdown that followed Morsi's ouster and saw thousands of Islamists detained.
Egypt has called on Qatar to respect a 1998 Arab counter-terrorism treaty and hand over Qaradawi and other wanted Islamists.
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