The accident occurred today morning on Interstate 380.
Monroe County Coroner Robert Allen told reporters at the scene that the death toll had climbed from two to three.
He says all the passengers on the bus were from Italy.
The tour bus has the name of the Academy Bus company but appears to have been operated by Viaggidea, an Italian tour operator whose name is also on the bus.
The company operates tours that head from New York to Niagara Falls and Canada.
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