Other participants in the series D funding included Sofina, Lightspeed Ventures and Times Internet Ltd.
The new round, which comes within months of a USD 75 million fund raise from Sequoia Capital and Sofina, will be deployed to fuel international expansion in countries like the US and the UK.
It already has a presence in the Middle East.
This is the first investment of CZI, which was founded by Facebook founder Mark Zuckerberg and his wife Priscilla Chan, in Asia.
BYJU's represents an opportunity to help even more students develop a love for learning and unlock their potential, he added.
The company had raised USD 9 million from Aarin Capital in 2013, followed by Series B funding of USD 25 million from Sequoia Capital last year.
The company is profitable in India with an month-on-month growth of 15 per cent.
"Revenues in the first five months of this financial year (FY2017) has already crossed the FY16 revenue of Rs 120 crore," the company said.
"Our vision closely aligns with their vision of advancing human potential and promoting equality," BYJU's founder and CEO Byju Raveendran said.
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