Harris, 30, was earlier confirmed to perform at the ceremony, which is slated to happen tonight, reported Us magazine.
"It's with deep regret that Calvin Harris will not be able to perform at the 2014 MTV EMA's this weekend due to illness. He sends his sincere apologies to all his fans," an MTV representative said.
The official Facebook page for the EMAs confirmed Harris' condition. "We have some really sad news. Calvin Harris has taken ill and will not be able to perform at tomorrow's show. He sends his sincere apologies. Get well soon, Calvin!" a post on the page read.
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